Clinical implications of chromatin accessibility in human cancers Biology Diagrams Individual sites display diverse patterns of interphase-to-mitosis dynamics in chromatin accessibility. (A) Example of DNase hotspots and peaks. (B) G1E + GATA1 DNase cut density profiles at the Gata2, Slc8b1, and Klf13 loci are shown to illustrate their spatial patterns. Broad versus narrow sensitivity patterns are captured by the hotspots
Despite chromosome condensation during mitosis visible by microscopy, the landscape of chromatin accessibility at the macromolecular level is largely unaltered. However, mitotic chromatin accessibility is locally dynamic, with individual loci maintaining none, some, or all of their interphase accessibility. Chromatin accessibility is generally perceived as a common property of active regulatory elements where transcription factors are recruited via DNA-specific interactions and other physico-chemical properties to regulate gene transcription. Recent work in the context of mitosis provides less trivial โฆ
Genome accessibility is widely preserved and locally modulated during ... Biology Diagrams
Although mitotic chromosomes are highly compacted and transcriptionally inert, some active chromatin features are retained during mitosis to ensure the proper postmitotic reestablishment of maternal transcriptional programs, a phenomenon termed "mitotic bookmarking." However, the dynamics and regula โฆ Studying chromatin accessibility, nucleosome positioning and TF behaviors in the context of mitosis therefore provides a unique perspective on how TFs interact with the chromatin. These studies call for cautious interpretation of chromatin accessibility results while providing rich hypotheses to its function in transcriptional control and its
During mitosis, transcription is globally attenuated and chromatin architecture is dramatically reconfigured. We exploited the M- to G1-phase progression to interrogate the contributions of the The dynamic regulation of chromatin accessibility is one of the prominent characteristics of eukaryotic genome. The inaccessible regions are mainly located in heterochromatin, which is multilevel compressed and access restricted. HNF4a and C/EBPฮฑ, as well as the cell cycle and mitosis repressive factor, E2F4, to the their target genes Mitosis is concomitant with global histone phosphorylation and deacetylation (), the loss of chromatin accessibility and long-range chromatin interactions (), and the dissociation of RNA polymerase II (Pol II) and transcription factors (TFs) from chromatin (3, 4), resulting in the silencing of gene transcription.Despite this, emerging observations have indicated that some chromatin features
